The Palme Group (Palmegruppen) within the police's criminal investigation unit reopened the hotline between 3pm and 10pm on Thursday and have reported that some new information has been gathered.
"We are now going to go through all the tips and see what can be new or not previously investigated information," said Dag Anderson at the Palme Group.
Palme was gunned down on Sveavägen as he and his wife walked home from a late night screening of the film The Mozart Brothers. The killing has spawned myriad conspiracy theories but no one has ever been jailed for the murder.
The Social Democrat prime minister was an outspoken critic of the war in Vietnam and apartheid in South Africa, among other international issues, as well as the leader of the workers movement in Sweden.
Last year, a documentary about him showed in Swedish cinemas, with a longer version split into a television series.
Also in 2012, the release of the feature film Call Girl divided opinion but brought Palme back into the limelight. Despite its director's claims that the film was fiction inspired by real events and the mood of a specific era in Swedish politics, many, including the Palme family, interpreted it as an accusation that Palme had bought sex.
The politician's death anniversary is usually honoured by his party, the Social Democrats, with their current leader Stefan Löfven placing a wreath on his grave in central Stockholm on Thursday morning.