"The people, who were out fishing, caught something, and on the hook was a human skull," police said in a statement.
The pair made the macabre discovery in the river Svartån, between Västerås and Skultuna, on Saturday afternoon.
The skull has been transported to a forensic medicine department in Uppsala where it will be tested further, but police said there was "the greatest likelihood" that it was a human skull.
Police have opened an investigation into murder and are carrying out further measures to determine whether a crime took place.
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